Modulation of adipogenesis-related gene expression by ethanol extracts of Detam 1 soybean and Jati belanda leaf in 3T3-L1 cells

Hidayat, Meilinah and Prahastuti, Sijani and Fauziah, Nurul and Maesaroh, Maesaroh and Balqis, Balqis and Widowati, Wahyu (2016) Modulation of adipogenesis-related gene expression by ethanol extracts of Detam 1 soybean and Jati belanda leaf in 3T3-L1 cells. Bangladesh Journal of Pharmacology, 11 (3). pp. 1-8. ISSN 1991-0088

Text 7. Meilinah, Sijani, Nurul, Maesaroh,Balqis&Wahyu_Modulation of Adipogenesis.......pdf - Published Version Restricted to Registered users only Download (453kB) | Request a copy

Abstract

In this study, we evaluated the effects of ethanol extracts of Detam 1 soybean, Jati belanda leaf, and the combination toward expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and stearoyl-CoA desaturase 1 (SCD1) genes in 3T3-L1 cells as anti-adipogenesis and anti-obesity. The differentiation of 3T3-L1 cells into adipocyte was conducted using induction medium consist of Dulbecco's Modified Eagle's Medium, 3-isobutyl-1-methylxanthine, insulin, dexamethasone, and fetal bovine serum. The expression of PPARγ, C/EBPα, and SCD1 gene was measured using real-time quantitative polymerase chain reaction (qPCR). Ethanol extract of Jati belanda at a concentration of 50 μg/mL was most effective to reduce PPARγ, C/EBPα, and SCD1 gene expression in 3T3-L1 cells. Ethanol extract of Detam 1 soybean failed to reduce PPARγ gene expression, whilst in the concentration of 50 μg/mL it was able to significantly reduce the C/EBPα and SCD1 gene expression. Both ethanol extracts of Detam 1 soybean and Jati belanda have potential as anti-adipogenesis and anti-obesity by suppressing adipogenesis-related gene expression, particularly C/EBPα and SCD1.

Item Type:	Article
Subjects:	R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Depositing User:	admin LPPM
Date Deposited:	08 Aug 2017 02:12
Last Modified:	10 Aug 2017 08:51
URI:	http://repository.lppm.maranatha.edu/id/eprint/47

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