Inhibitory Potential of Ethanol Extract of Detam 1 Soybean Glycine max)Seed and Jati Belanda (Guazuma ulmifolia)Leaves on Adipogenesis and Obesity Models in 3T3-L1 Cell Line

Hidayat, Meilinah and Soeng, Sylvia and Prahastuti, Sijani and Erawijantari, Pande Putu and Widowati, Wahyu (2015) Inhibitory Potential of Ethanol Extract of Detam 1 Soybean Glycine max)Seed and Jati Belanda (Guazuma ulmifolia)Leaves on Adipogenesis and Obesity Models in 3T3-L1 Cell Line. Journal of Scientific Research & Reports 6 ( 4 ): 304 - 312 , 20 15 ; Article no.JSRR.20 15 . 156, 6 (4). pp. 1-9. ISSN 2320 - 0227

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Abstract

Obesity is considered an emergency health problem through out the world and is characterized by an increase in the number and size of adipocytes in adipose tissue. Some phytochemical bioactive have been shown to inhibit adipocyte differentiation and induce adipocyte apoptosis. The Objective: In this ex vivo study, we evaluated the cytotoxic effects, antiobesity and antidiapogenesis potential of ethanol extract of Detam 1 soybean seed (EEDS), Jati Belanda leaves (EEJB) and their combinations on 3T3-L1 cells. Experimental Approach: The cytotoxic effect of EEDS and EEJB were assayed using MTS assay. Triglyceride (TG) level and inhibitory activity were assayed using a TG assay kit. Glucose 6- phosphate dehydrogenase (G6PD) activity and inhibitory activity were determined by using G6PDH assay kit. The cholesterol level was measured according to the Chol Kit Randox protocol. Results: The lowest cytotoxic activity and safe substances on 3T3-L1 cell were EEDS and EEJB in 50 and 10 μg/ml of concentration. EEJB in the concentration of 50 μg/ml was the most active to inhibit G6PD, TG, and cholesterol activity with inhibition activities 47.30%, 37.93% and 73.91% respectively compared to the control (differentiated 3T3-L1 adipocyte). Conclusion: Ethanol extract of Detam 1 soybean seed and Jati Belanda leaves posses the inhibitory potential on G6PD, triglyceride and cholesterol activities in 3T3-L1 cell line and the most active compound showed by ethanol extract of Jati Belanda leaves.

Item Type:	Article
Uncontrolled Keywords:	Obesity; adipogenesis; Detam 1 soybean; Jati belanda; 3T3-L1; G6PD; triglyceride;cholesterol.
Subjects:	R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Kedokteran
Depositing User:	admin LPPM
Date Deposited:	08 Aug 2017 02:08
Last Modified:	10 Aug 2017 08:49
URI:	http://repository.lppm.maranatha.edu/id/eprint/45

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