β-Conglycinin Content Obtained from Two Soybean Varieties Using Different Preparation and Extraction Methods

Hidayat, Meilinah and Sujatno, Muchtan and Sutadipura, Nugraha and S., Setiawan and Faried, Ahmad (2011) β-Conglycinin Content Obtained from Two Soybean Varieties Using Different Preparation and Extraction Methods. HAYATI Journal of Biosciences March 2011, 18 (1). pp. 1-6. ISSN EISSN:2086-4094

Text 2. Meilinah,Muchtan,Nugraha,Setiawan&Ahmad_β Conglycinin Content Obtained from Two Soybean Varieties Using Different Preparation and Extraction Methods.pdf - Published Version Restricted to Registered users only Download (74kB)

Abstract

Soybean is a good source of protein. It has two major fractions, β-conglycinin (7S) and glycinin (11S). β- conglycinin’s function was known to suppress food intake, and this effect may be due to stimulating endogenous cholecystokinin (CCK) release. The aims of this study were to determine the highest content of total β-conglycinin and β-conglycinin sub unit-β level obtained from two varieties of soybean i.e. Wilis and Detam 1 varieties using different preparation and extraction methods. These two soybean varieties were prepared into tempeh. Then the seed and tempeh were extracted using Deak and Panthee methods. There were six extracts analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) and coomassie brilliant blue (CBB) staining. The result was shown that Detam variety and raw seed contained the highest total β-conglycinin level. And Panthee method was the best method for extraction of total β-conglycinin, while Deak method was the best method for extraction of β-conglycinin subunit-β.

Item Type:	Article
Uncontrolled Keywords:	Detam 1 soybean, Wilis soybean, Deak method, Panthee method, SDS PAGE, CBB staining
Subjects:	R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Kedokteran
Depositing User:	admin LPPM
Date Deposited:	14 Aug 2017 03:52
Last Modified:	14 Aug 2017 03:52
URI:	http://repository.lppm.maranatha.edu/id/eprint/165

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