Protein extract of Detam 1 soybean contains β–conglycinin stimulating cholecystokinin signal transduction through activities of PKC, c-RAF and ERK 1/2 in Wistar rats

Hidayat, Meilinah and Faried, Ahmad (2015) Protein extract of Detam 1 soybean contains β–conglycinin stimulating cholecystokinin signal transduction through activities of PKC, c-RAF and ERK 1/2 in Wistar rats. BIOMEDICAL ENGINEERING, 1 (1). pp. 1-7. ISSN 2356-2471

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Abstract

Objective : to determine which kind of soybean extract contains the highest β–conglycinin level, their effect cholecystokinin (CCK)plasma level, and to signal transduction pathway in duodenum of Wistar rats. Methods: β-conglycinin were analysed in vitro using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) and coomassie brilliant blue (CBB) staining. The CCK plasma level were measured in vivo in 24 male Wistar rats by ELISA method, using t-test paired and the activity of PKCμ, PKA, c-Raf and ERK in CCK signal transduction pathway in vivo by Western blot method and Scion image densitometry (DU) analysed using ANOVA. Result: Protein extract of Detam 1 soybean (PEDS) contains the highest amount of β-conglycinin level. There was no significant diffrerence of CCK plasma level between pre and post treatment (p> 0.05) although there were increasing level in all treatment. The duodenum of Wistar rats which were given PEDS showed significant difference with negative control in signal transduction activities of PKCμ, c-Raf & ERK 1/2 pathway (p<0.001). Conclusion: PEDS contains the highest β-conglycinin level, effects to CCK plasma level, and to CCK signal transduction pathway through activities of PKCμ, c-Raf and ERK 1/2 in enteroendocrine cells in duodenum Wistar rats

Item Type:	Article
Uncontrolled Keywords:	β conglycinin Cholecystokinin Protein Extract of Detam 1 soybean Signal transduction
Subjects:	R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Kedokteran
Depositing User:	admin LPPM
Date Deposited:	08 Aug 2017 07:26
Last Modified:	11 Aug 2017 00:56
URI:	http://repository.lppm.maranatha.edu/id/eprint/141

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